TABLE 2.
Primers
| ORF name or description | PFGRC locus no.a | Template strain | Primer 1 | Primer 2 | Product length (bp) | Reference |
|---|---|---|---|---|---|---|
| seh | NA | MW2 | ATTACTTTCATTCACATCATATGC | GTCACATTTTTCTTATTTGTTCG | 431 | This study |
| sej | NA | U1 | TTGCAATATGTAGTGTAAACAATGC | CAGCTACTCCTATATTTTTAGGCTCG | 421 | This study |
| sarT | SA2506 | RN6390 | GTAAGGGATGAACTCGAGATGAATGATT | ACGGGGATCCAAAAATACATTTAACTGC | 420 | 61 |
| sarU | SA2507 | RN6390 | TGACGATTTCGGCTGAACTTC | TGGAACACGAAATGGTGAAC | 2,300 | 39 |
| trap | SA1891 | RN6390 | CGCGCGGATCCCAACTATTCCAATTTTCAG | CGCGAAGCTTCTTAAAGTCTTCGTATG | 317 | 3 |
| cna | NA | U1 | CAAGCAGTTATTACACCAGACGG | CACCTTTTACAGTACCTTCAATACC | 688 | This study |
| sdrE | SA0610 | SANGER476 | CAGTAAATGTGTCAAAAGA | TTGACTACCAGCTATATC | 767 | 49 |
| bbp | NA | SANGER252 | CAGTAAATGTGTCAAAAGA | TACACCCTGTTGAACTG | 1,055 | 49 |
| clfB | SA2652 | U1 | TGCAAGATCAAACTGTTCCT | TCGGTCTGTAAATAAAGGTA | 596 | 49 |
| epiC | SA1876 | COL | CAAAAGCATCTACTGAAACC | CAACGTCGTCATTACACC | 779 | This study |
| isaA | SA2660 | COL | TTGACTTAGCGCATAATCACC | TTAGAATCCCCAAGCACC | 584 | This study |
| isaB | SA2584 | COL | GTGTAGCAGCAACATTAGC | TATTATGATCAACGACAAACC | 456 | This study |
| LPXTG motif protein | SA2676 | COL | CCTACAAATAACACGTTCC | GCACTATCGTAACTTAATCC | 602 | This study |
| lukD | SA1880 | COL | CAATTGCACTGCTTTTGC | ATTTTGATCCATTTAATCCACC | 447 | This study |
| Transcriptional regulator, GntR family | SA0091 | COL | ACTTATAGCGAAAGACATAATATTCC | AGACTAACCATACAAAATAACCTCC | 415 | This study |
| DNA-binding response regulator, putative | SA2646 | COL | TGATGATATACTGTCTATCTTTAACC | CATCTATGAAGTTTTCTGATTCC | 464 | This study |
| aur | SA2659 | COL | TTACGCATTATACATTGCAACC | CCAAGTACACCTTTTCCTTTACC | 413 | This study |
| lukM | SA2006 | COL | AGATAAAAATTCAACAGCACC | ACCATACTTCAAGTCATTCG | 514 | This study |
| sdrD | SA0609 | U1 | GGAAATAAAGTTGAAGTTTC | ACTTTGTCATCAACTGTAAT | 500 | 49 |
| ABC transporter, ATP-binding protein | SA1893 | COL | AAGTAGAACAGCTTACAGGTGG | CGGGATTAACGATAAACG | 447 | This study |
| LPXTG-motif protein | SA0119 | COL | CAAAATGTACAAAATCATCGTCC | CGTCGTCTAAATGCTAAGAATAACC | 590 | This study |
| fnbA | SA2511 | U1 | ATCTTAACTTTTCATTAACTCGCT | AATTTCCTCGACTGGTCCTTGTGC | 2,200 | 64 |
| fnbB | SA2509 | COL | ATGTTGAAACTCATGGTATCTCAAC | GAACGCCTTCATAGTGTCATTGAG | 2,277 | 64 |
a
NA, none available.