TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| N. uniformis strain | ||
| ATCC 21806 | Wild type | 2 |
| nocK::apr | Inactivation of nocK by insertion of apr | This work |
| nocL::apr | Inactivation of nocL by insertion of apr | This work |
| E. coli SC12155 | Supersensitive to β-lactam antibiotics | 2 |
| Plasmid | ||
| pULVK2 | Bifunctional E. coli-Nocardia plasmid, Kmr | 27 |
| pULVK2/KAm | Derivative of pULVK2 containing the nocK fragment with apr insertion | This work |
| pULVK2/LAm | Derivative of pULVK2 containing the nocL fragment with apr insertion | This work |
| pET24b/nocL | Derivative of pET24b(+) containing the native sequence for nocL | This work |
| pIJ4070/nocL | Derivative of pIJ4070 containing nocL behind ermE2 | This work |
| pULVK2T | Derivative of pULVK2, Kmr and Tsr | 11 |
| pULVK2T/nocL | Derivative of pULVK2T containing nocL behind control of the ermE2 promoter | This work |