TABLE 3.
Effect of the DSR on transcription from pvuIIM promoter M82
| pvuIIC allelea | DSRb | CAT activityc
|
|
|---|---|---|---|
| Absolute | Normalized | ||
| WT | + | 1,202 ± 118 | 1.00 |
| − | 3 ± 0.5 | <0.01 | |
| Esp19 | + | 1,183 ± 440 | 0.98 |
| − | 3 ± 0.5 | <0.01 | |
a
C.PvuII is supplied in trans from either the WT allele or a mutant inactive allele in which an extra codon has been inserted within the predicted HTH motif.
b
Plus and minus signs indicate the presence and absence of the DSR, respectively.
c
CAT activity was measured as described in Materials and Methods and is expressed as mean counts per minute per minute incorporated per microgram of protein ± the standard error from triplicate samples. The vector control levels (not shown) were subtracted as background.