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Immunohistochemical double staining of heparin-binding EGF-like growth factor (HB-EGF, DAB) and macrophages (CD68, PermaBlue), without nuclear counterstaining (A), and the simulated fluorescence composite image obtained through spectral imaging (B), showing not only macrophages (arrow), but also other cells in the stroma and tumor cells strongly expressing HB-EGF in cervical cancer.
