Figure 4.

Kallistatin inhibits tube formation of hLEC cells. (A) Representative microscope images of hLEC cells. The cells (plated on Matrigel) were treated with 640 nM rKAL or PBS for 12 h; ×100 magnification. The histogram represents the tube numbers per field. (B) hLECs were transfected with Ad-GFP/Ad-KAL for 48 h and then plated on Matrigel for 12 h; tube numbers were counted; scale bar, 50 µm. ^*P<0.05, ^**P<0.01, the results are presented as the mean ± standard deviation. hLECs, human lymphatic endothelial cells.