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. 2017 Apr 20;50(6):2000–2010. doi: 10.3892/ijo.2017.3972

Figure 7.

Figure 7

Kallistatin inhibits the VEGFR-3/ERK and VEGFR-3/Akt signaling pathways in hLECs cells. (A) After transfection with Ad-GFP/Ad-KAL for 24 h, cells were harvested for immunoblotting analysis of VEGFR-3, p-ERK/ERK and p-Akt/Akt expression levels. (B) Histograms representing the expression levels of VEGFR-3/actin, p-ERK/ERK and p-Akt/Akt. (C) Immunofluorescence assay was performed to detect p-ERK in hLECs treated with 640 nM rKAL for 24 h. (D) Immunofluorescence assay was performed to detect p-Akt in hLECs treated with 640 nM rKAL or Ad-KAL for 24 h. Scale bar, 50 µm. *P<0.05, **P<0.01. Results are presented as the mean ± standard deviation.