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. 2017 May 8;50(6):1977–1988. doi: 10.3892/ijo.2017.3985

Figure 1.

Figure 1

Cx32 is aberrantly expressed and localized in cell lines and correlates with cell invasiveness. (A) qRT-PCR assay was performed to detect the mRNA level of Cx32 in human normal hepatic cell line LO2 and HCC cell lines HepG2, SMMC-7721 and Huh7, respectively. (B) Western blot analysis was conducted to detect the protein level of Cx32 in the above cell lines. (C) Immunofluorescence assay was used to detect the expression and localization of Cx32 in LO2 and SMMC-7721 cells (original magnification, ×400). Both membranous (marked by red arrow) and cytoplasmic (marked by white arrow) localization of Cx32 was observed in LO2 cells, while SMMC-7721 cells displayed a decreased Cx32-specific positive spots, predominantly located in the cytoplasm. (D) Transwell invasion assays of LO2, HepG2, SMMC-7721 and Huh7 cells (original magnification, ×400). Data represent the mean ± SEM of three to five independent experiments. *P<0.01 vs. LO2; #P<0.01 vs. SMMC-7721.