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. 2017 May 8;50(6):1977–1988. doi: 10.3892/ijo.2017.3985

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Modulation of Cx32 in HCC cells influences EMT or MET process in vitro. (A) Cell morphological changes of Cx32 downregulated SMMC-7721 and Cx32 overexpressed HepG2 cells as observed by microscopy. Images are representative of three independent experiments (original magnification, ×200). (B) Effect of Cx32 on expression of EMT-related proteins. E-cadherin and vimentin were examined in Cx32 downregulated SMMC-7721 and Cx32 overexpressed HepG2 cells by western blot analysis. (C) Immunofluorescence assay was performed to assess the expression of markers of epithelial and mesenchymal phenotypes (original magnification, ×400). NC, negative control; OE, overexpression.