FIG. 4.
Effect of minD mutations on the ability of MinD to activate MinC to inhibit colony formation. All plasmids were derivatives of pCS104CD, which carries minC and minD under the control of the PBAD promoter on a low-copy-number plasmid. Colonies of JS964 (Δmin) carrying the various plasmids were picked and resuspended in L broth. The cultures were serially diluted (10-fold each step), and 5 μl was spotted on plates carrying various arabinose concentrations. The minD mutation carried on the plasmid is indicated to the right of the figure. From the top, the first is wild-type (WT) minD, the second contains the minE gene downstream of minD, the third contains minDΔ10 (missing the carboxy-terminal 10 amino acids), and the others carry the mutations isolated in this study.