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Localization of MinE-GFP in the presence of D152A. MinE-GFP was localized in the absence of MinD (A), the presence of MinD (B), or the presence of D152A (C). JS964 containing pJK110 (minE-gfp), pJK100 (minDE-gfp), or pJK100A (minDD152AE-gfp) was grown in L broth and induced with 20 μM IPTG.
