TABLE 1.
Description of FURTA-positive chlamydial genomic plasmid inserts
| Plasmid designation | Insert size (bp) | Presence of ≥2 separate regionsa | Target ORF(s) (I or II)b | Descriptionc |
|---|---|---|---|---|
| pJER20 | 430 | Yes | CT089 lcrE (I) (sctW copN) | Regulatory protein, type III secretion |
| CT028 rl19 (II) | Ribosomal protein | |||
| pJER45 | 223 | Yes | CT261 dnaQ_I (I) | DNA polymerase III alpha or epsilon chain |
| pJER61 | 37 | No | CT529 (I) | Chlamydia-specific hypothetical protein |
| pJER63 | 420 | Yes | CT558 lipA (I) | Lipoic acid synthase |
| pJER115 | 771 | Yes | CT737 yccJ (I) | SET domain DNA binding protein |
| pJER120 | 62 | No | CT504 (II) | Chlamydia-specific hypothetical protein |
| pJER123 | 237 | No | CT733 (I) | Chlamydia-specific hypothetical protein |
| CT734 (I) | ||||
| pJER177 | 724 | Yes | CT077 apbE (I) (yojL) | Inner membrane lipoprotein |
| pJER178 | 359 | Yes | CT709 mreB (I) | Shape-determining protein |
| pJER179 | 739 | No | CT634 nqrA (I) | NADH-ubiquinone oxidoreductase |
| pJER180 | 646 | No | CT817 tyrP_1 (I) | Tyrosine/tryptophan transport protein |
| pJER229 | 782 | Yes | CT603 aphC (I) | Antioxidant peroxidase |
| pJER230 | 864 | Yes | CT077 apbE (I) (yojL) | Inner membrane lipoprotein |
| pJER233 | 771 | Yes | CT769 ybeB (I) | Hypothetical protein |
| CT770 fabF (I) | Beta-ketoacyl ACP-synthase | |||
| CT414 pmpC (II) | Outer membrane protein | |||
| pJER235 | 84 | No | CT556 (II) | Chlamydia-specific hypothetical protein |
| pJER268 | 178 | No | CT770 fabF (I) | Beta-ketoacyl ACP-synthase |
| pJER291 | 787 | No | CT492 yacE (I) | Probable ATPase or kinase |
| pJER369 | 846 | Yes | CT152 ycfV (II) | ABC transporter ATPase |
| CT758 murD (I) | N-Acetylmuramyl l-alanine d-glutamate ligase | |||
| pJER373 | 394 | No | CT015 phoH (II) | Phosphate starvation-inducible ATPase |
| CT016 (I) | Chlamydia-specific hypothetical protein | |||
| pJER391 | 66 | No | CT736 ybeL (I) | Hypothetical protein |
| pJER406 | 186 | No | CT248 glgP (I) | Alpha glycan phosphorylase |
| CT249 (I) | Hypothetical protein | |||
| pJER408 | 211 | No | CT597 tolR (II) | Transport protein |
| CT598 (I) | Chlamydia-specific hypothetical protein | |||
| pJER435 | 581 | No | CT709 mreB (I) | Shape-determining protein |
a
The plasmid insert contains two or more genomic fragments that have ligated together from separate, noncontiguous regions of the C. trachomatis chromosome.
b
Target ORFs in group I indicate that the FURTA-positive sequence is less than 300 bp upstream of the predicted translational start codon; target ORFs in group II indicate that the FURTA-positive sequence is less than 100 bp downstream of the predicted translational start codon.
c
ACP, acyl carrier protein.