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. 2005 Jan;187(2):639–648. doi: 10.1128/JB.187.2.639-648.2005

FIG. 1.

FIG. 1.

Pellicle and biofilm formation in SOBG. The pellicles are thick aggregates of cells on the culture surface. The biofilms are aggregates of cells around the edge of the culture tube at the air-liquid-surface interface. (A) Pellicles formed in Ech 3937 SOBG cultures grown at 25°C (tube 1), but only biofilms and not pellicles formed in cultures grown at 36°C (tube 2). (B) All known TTSS regulatory genes were required for wild-type pellicle, but not biofilm, formation. The hrpL mutant WPP96 is shown. Tube 3, Ech 3937; tube 4, WPP96; tube 5, WPP96(p50hrpL). (C) The hrpS and hrpL genes expressed on plasmids restored pellicle formation to the hrpX hrpS mutant WPP89. Tube 6, Ech 3937; tube 7, WPP89; tube 8, WPP89(p105S); tube 9, WPP89(p50hrpL). (D) TTSS structural genes were also required for pellicle, but not biofilm, formation. Tube 10, Ech 3937; tube 11, WPP100; tube 12, WPP100(p415hrpA).