TABLE 1.
Primers used in this studya
| No. | Primer set | Sequence (5′→3′) (Restriction enzyme sites) | Targeted region |
|---|---|---|---|
| 1a | hrpY-D1 | TGATGGCCAGCATTTCGGC | 2.8-kb hrpY-hrpL |
| 1b | hrpL-f1 | AAGGATCCGATGGAGAGTTAATGAAATG (BamHI) | |
| 2a | hrcJ-D1 | ACCAGGGCTTGCCGAAACGC | 3.4-kb hrcJ-hrpY |
| 2b | hrpY-C1 | GGAAGCTTCGCAGAGTACGCTGATGATCGCTGACGATC (HindIII) | |
| 3a | hrpY-D1 | TGATGGCCAGCATTTCGGC | 3.8-kb hrpY-hrpJ |
| 3b | hrpJ-A1 | TCGTTGCAGCGTACGCAGCC | |
| 4a | hrpL-B2 | GCGTACTCTGCGAAGCTTCCTCCACGGTATTGGCAGGGGT (HindIII) | 2.0-kb hrpL-hrcV |
| 4b | hrcV1 | CCGAACGCCTCCACCACGTG | |
| 5a | hrcJ-D1 | ACCAGGGCTTGCCGAAACGC | 3-kb hrcJ-hrpS |
| 5b | hrpS-f1 | GAGCTCGGATCCAGCGTTTAATCGCCTGA (SacI, BamHI) | |
| 6a | hrpY-C1 | GGAAGCTTCGCAGAGTACGCTGATGATCGCTGACGATC(HindIII) | 3-kb hrpY-hrpA |
| 6b | hrpA-r1 | GTCGACTACCTAGAATTCATCAG (EcoRI) | |
| 7a | hrpXY-f1 | GAGCTCGGATCCGCCAGGCTGTTGATGC (SacI, BamHI) | 2.5-kb hrpX-hrpY |
| 7b | hrpXY-r1 | ACGAAGGGAGGACTCTAGAC (XbaI) | |
| 8a | hrpY-FBamHI | TCTGGAGGAGGATCCGCGAATG (BamHI) | 0.7-kb hrpY |
| 8b | hrpY-RHindIII | TACAAGCTTTCATCAGGCGA (HindIII) | |
| 9a | hrpS-f1 | GAGCTCGGATCCAGCGTTTAATCGCCTGA (SacI, BamHI) | 1.45-kb hrpS |
| 9b | hrpS-r1 | ACAGCCCGATAAATCTAGAGCC (XbaI) | |
| 10a | hrpL-f1 | AAGGATCCGATGGAGAGTTAATGAAATG (BamHI) | 0.55-kb hrpL |
| 10b | hrpL-r1 | TTGAATTCTTATGCATCAACGGCCTGGC (EcoRI) | |
| 11a | hrcJ-f1 | TGCACTAGTCAGGATCAAACCC (SpeI) | 0.97-kb hrcJ |
| 11b | hrcJ-r1 | CATCGGCTACACGTAGAATTCTCA (EcoRI) | |
| 12a | hrpA-f1 | ATCGGGCTGTATACTAGTATCACC (SpeI) | 0.3-kb hrpA |
| 12b | hrpA-r1 | GTCGACTACCTAGAATTCATCAG (EcoRI) | |
| 13a | bcsA-f1 | ACCCGAATTCCTATATTCGCCGTCACC (EcoRI) | 3-kb bcsA |
| 13b | bcsA-r12 | CCCACGCACAGGGACAACA | |
| 14a | RT-hrpLf1 | GGGTATTTGGACTTGCCCTGAATC | 223-bp hrpL |
| 14b | RT-hrpLr2 | GCATCCAGCAGCATCATCAACATC | |
| 15a | hrpN1 | GCGGCATGGCGAAAGAGAT | 226-bp hrpN |
| 15b | hrpN2 | GTGTTGCCGGTATCACCC | |
| 16a | RT-rplUf1 | GTTTGACCAGGTTCTGATGGTTGC | 162-bp rplU |
| 16b | RT-rplUr1 | CCAGCCTGCTTACGGTAGTGTTTA |
a
Primer sets 1 through 13 were used to amplify hrp and hrc genes for construction of plasmids used for allelic exchange mutagenesis and complementation experiments. Primer sets 14 through 16 were used for RT-PCR. Restriction sites in primers are in boldface type.