TABLE 1.
B. subtilis strains used in this studya
| Strain | Genotype | Source or reference and/or description |
|---|---|---|
| 168 | trpC2 | C. Anagnostopoulos |
| HH443 | trpC2 amyE::[pLJ25 xpt′-lacZ (neo)] pbuE1 ydhK::pBOE335 (cat) | LJ26 transformed with DNA from LJ40 selecting for Cmr |
| KN05n | trpC2 amyE::[pKN05n glyA′-lacZ (neo)] | 10 |
| LJ24 | trpC2 amyE::[pLJ24 pbuE′-lacZ (cat)] | 4 |
| LJ25 | trpC2 amyE::[pLJ25 xpt′-lacZ (neo)] | 4 |
| LJ26 | trpC2 amyE::[pLJ25 xpt′-lacZ (neo)] pbuE1 | 4 |
| LJ27 | trpC2 thr-5 pbuE1 | 4; 2-fluoroadenine resistant |
| LJ32 | trpC2 pbuE::pLJ32 (erm) | 168 transformed with pLJ32 selecting for Err. pLJ32 is pMutin4 (16) containing an internal part of the ydhL transcriptional unit |
| LJ40 | trpC2 ydhK::pBOE335 (cat) | 168 transformed with pLJ40 selecting for Cmr. pLJ40 is pBOE335 (9) containing an internal part of the ydhK transcriptional unit |
| LJ54 | trpC2 amyE::[pLJ25 xpt′-lacZ (neo)] pbuE::pLJ42 (cat) | 4 |
| ED265 | trpC2 ade-1 | 8 |
| ED279 | trpC2 ade-1 apt-7 | 8 |
| ED181 | trpC2 purF6 pbuG1 | 11 |
| ED182 | trpC2 purF6 | 11 |
| ED463 | trpC2 purF6 pbuG1 ydhK::pBOE335 (cat) pbuE1 | ED181 transformed with DNA from HH443 selecting for Cmr |
| ED464 | trpC2 purF6 ydhK::pBOE335 (cat) pbuE1 | ED182 transformed with DNA from HH443 selecting for Cmr and 2-fluoroadenine resistance |
| ED501 | trpC2 amyE::[pLJ24 pbuE′-lacZ (cat)] ade-1 | ED265 transformed with DNA from LJ24 selecting for Cmr |
| ED502 | trpC2 amyE::[pLJ24 pbuE′-lacZ (cat)] ade-1 apt-7 | ED279 transformed with DNA from LJ24 selecting for Cmr |
| ED503 | trpC2 pbuE::pLJ32 (erm) ade-1 | ED265 transformed with DNA from LJ32 selecting for Err |
| ED504 | trpC2 pbuE::pLJ32 (erm) ade-1 apt-7 | ED279 transformed with DNA from LJ32 selecting for Err |
| ED508 | trpC2 amyE::[pKN05n glyA′-lacZ (neo)] pbuE::pLJ42 (cat) | KN05n transformed with DNA from LJ54 selecting for Cmr |
| ED509 | trpC2 thr-5 amyE::[pKN05n glyA′-lacZ (neo)] pbuE1 | LJ27 transformed with DNA from KN05n selecting for Neor |
a
The designation ydhL has been replaced with the novel functional designation pbuE, defined in reference 4. For selection of antibiotic resistance, antibiotics were used at the following concentrations: ampicillin, 100 mg/liter; neomycin (Neo), 5 mg/liter; erythromycin (Er), 1 mg/liter; lincomycin, 25 mg/liter; and chloramphenicol (Cm), 6 mg/liter. Isolation of DNA and basic molecular biology techniques were performed as previously described (9).