Fig. 6. B10G5 cotargeting sMIC during CTLA4 blockade therapy increases T cell content and TCR clonality in tumors.

DNA were isolated from paraffin-embedded tumor sections and subjected to immunoSEQ assay, as previously described (Adaptive Biotechnologies). Tumor in situ TCR abundance (A) and clonality (B) were assayed by surveying TCR3β expression and analyzed with the Shannon diversity index using immunoSEQ Analyzer software. (C) Representative IHC confirming increased CD8 T cell infiltration in tumors with B10G5 cotargeting sMIC.