Figure 6.

SPT5 and SPT16 Associate with RNAPII-Transcribed Loci, but Not with Loci Transcribed by RNAPI and RNAPIII.

SPT5 and SPT16 are enriched at genes transcribed by RNAPII (orange), but not at loci transcribed by RNAPI (blue) and RNAPIII (green), or at nontranscribed regions (gray). ChIP analyses of various loci transcribed by different RNA polymerases. The antibodies used are directed against SPT5 (A), SPT16 (B), RNAPII-CTD-S2P/S5P ([C] and [D]), histone H3 (E), or without antibody (F). For the ChIP experiments, percentage input was determined by qPCR, and P values for all qPCR data were calculated using two-sample t tests (unpaired and two-tailed). Error bars indicate sd of at least three biological (independently pooled collections of tissue) and three technical replicates (independent qPCR measurements each). Data sets marked with asterisks are significantly different from TA3 (gray): *P < 0.05, **P < 0.01, and ***P < 0.001.