Figure 5.

Abundance of IPA1 and Expression Levels of Downstream Genes Are Modulated by Overexpressing IPI1.

(A) and (B) An in vitro degradation assay was performed in shoot apexes (A) and young panicles (B) to detect the IPA1 protein. The samples were collected as indicated and relative amounts of proteins were determined by densitometry normalized to actin.

(C) IPA1 abundance in shoot apexes in Nipponbare (NP) and IPI1-overexpressing transgenic (Ub:IPI1) plants. Relative amounts of IPA1 protein were determined as (A) and expressed relative to the value of Nipponbare.

(D) and (E) Quantitative real-time PCR was performed to detect the transcript levels of DEP1 (D) and OsTB1 (E) in shoot apexes in NP and Ub:IPI1. Double asterisk indicates P < 0.01 (Student’s t test, n = 3), and bars indicate sd.

(F) IPA1 abundance in young panicles in NP and Ub:IPI1. The proteins were detected as in (C).

(G) and (H) Quantitative real-time PCR was performed to detect the transcript levels of DEP1 (G) and OsTB1 (H) in young panicles in NP and Ub:IPI1. Double asterisk indicates P < 0.01 (Student’s t test, n = 3), and bars indicate sd.