TABLE 2.
Primer information
| Primer no. | Primer nucleotide sequencesa | Enzyme sites |
|---|---|---|
| P17 | 5′-AGTAAAGCTTATGATGCTGTTTCCAGTA-3′ | HindIII |
| P18 | 5′-AGTAGGATCCTGAAATGTAGGGGCCCGG-3′ | BamHI |
| P35 | 5′-AGTACTCGAGGAATCCAGACGTGAGGCT-3′ | XhoI |
| P36 | 5′-AGTAGGTACCTCGTCGGACCTGATTGTG-3′ | KpnI |
| P37 | 5′-AGTATCTAGAATCGCCCGATTGCAGCGG-3′ | XbaI |
| P38 | 5′-AGTAGGATCCTCCTGCAGCTTGCGCAGT-3′ | BamHI |
| P46 | 5′-ATGCGGATCCATGCGCCTTCGCAATCCC-3′ | BamHI |
| P101 | 5′-ATGCGGATCCTCAATGCGCCCTGTCAAT-3′ | BamHI |
| P128 | 5′-ATCGAAGCTTGGCAACTCACCATGCGTA-3′ | HindIII |
| P129 | 5′-AGTCGAATTCATGCGCCTTCGCAATCCC-3′ | EcoRI |
| P132 | 5′-AGTAGTCGACTCACGAGACAACAACGTC-3′ | SalI |
| P133 | 5′-AGTAGGATCCTCGGAAGGTCGGGACCAC-3′ | BamHI |
| P139 | 5′-ATGCTCTAGAGTGTGGCATCGCGCTGAA-3′ | XbaI |
| P140 | 5′-ATGTGGATCCGGTACTTTGGAATCGGGG-3′ | BamHI |
| P141 | 5′-ACGCGAATTCTGAGGGCGGACCGTCCAT-3′ | EcoRI |
| P142 | 5′-ATTCGGTACCACCTCAGTAGCGAGGCCG-3′ | KpnI |
| P209 | 5′-TGCGTCTAGAGAGCTGCTTTTGTTCCTG-3′ | XbaI |
| P210 | 5′-ATGTGGATCCAGGAGTGGGTTGGTTCAC-3′ | BamHI |
| P219 | 5′-AGCGAAGCTTGCTGTAAGACTTGGGTTT-3′ | HindIII |
| P224 | 5′-GCACCTCGAGTGGAACCAACTTGCACCT-3′ | XhoI |
| P225 | 5′-ATGTGGATCCTCACTTGTCATCGTCGTC-3′ | BamHI |
| P226 | 5′-ATGTGGTACCATGCGCCTTCGCAATCCC-3′ | KpnI |
| P277 | 5′-ATGCGGATCCTGCCTGAGTGGTTATCTG-3′ | BamHI |
| P278 | 5′-ATGCGGATCCCTGTTTCCTGTGTGAAAT-3′ | BamHI |
| P279 | 5′-ATGCGAATTCCACATTTCCCCGAAAAGT-3′ | EcoRI |
| P313 | 5′-AGTAAAGCTTTGAAAATGTCGGGGCCCGG-3′ | HindIII |
| P316 | 5′-AGTAGGATCCGACCTTATAGGAAAGCCT-3′ | BamHI |
| P318 | 5′-AGTAGGATCCCACGAGACGGGCGGTTCA-3′ | BamHI |
| P319 | 5′-AGTAAAGCTTCACGAGACGGGCGGTTCA-3′ | HindIII |
| P320 | 5′-AGTATCTAGACTTAGCGGTAGAGCATTG-3′ | XbaI |
| P323 | 5′-AACAAGATCGTCTACGTA-3′ | |
| P324 | 5′-GCTTGCAGACGACTGAAA-3′ | |
| P325 | 5′-TCTCGAAGGAATGGAGCA-3′ | |
| P326 | 5′-CGTGAAGATGCATTTCGC-3′ | |
| P327 | 5′-TCACGAGACAACAACGTC-3′ | |
| P331 | 5′-CGCGCGTATAAAAACCTG-3′ | |
| P332 | 5′-CTTTTTAGCGGCAGACGG-3′ | |
| P333 | 5′-ATGGGATTTTTACGTCCG-3′ | |
| P334 | 5′-TTACAGGCGCACCTCTCC-3′ | |
| P338 | 5′-ATGATGCTGTTTCCAGTA-3′ | |
| P339 | 5′-CGCGGAGATTCAATCATG-3′ | |
| P340 | 5′-AACTTGTGCTCGTTACGC-3′ | |
| P350 | 5′-CCAGAACCTGTTTCAGAT-3′ | |
| P355 | 5′-GCATGGTACCCATCAGGATCGCCAGATG-3′ | KpnI |
| P356 | 5′-GCATCTCGAGCATAACGTTGATCCGGTG-3′ | XhoI |
| P366 | 5′-ATGAAAGCTTGGAGGCCTAATCATGCACGAGACGGGCGGTTCA-3′ | HindIII |
| P391 | 5′-AGCTGGATCCTTAGCGGTAGAGCATTGC-3′ | BamHI |
| P562 | 5′-AGTCGAATTCAACAATGCGTATATCCAGTTCTC-3′ | EcoRI |
| P638 | 5′-CACCACTCTCGGAAGGCAACTAACAATGCGT-3′ | |
| P639 | 5′-ATGCGCCTTCGCAATCCCGAA-3′ | |
| P754 | AGTCGGATCCTCATGCATAATCAGGTACATCATAAGGATAATGCGCCTTCGCAATCCC | BamHI |
| P1073 | 5′-ATGAGGATCCACCGCTGTAAGACTTGGG-3′ | BamHI |
| P1245 | 5′-CACCCGTACGCGGAGATTCAACAATGAGACC-3′ | |
| P1246 | 5′-GACCTTATAGGAAAGCCTCACGTCTGG-3′ | |
| P1281 | 5′-GGGCGTCTGGGCGGACAGATGGATGCTGTGAAAAAGCATAAGGC-3′ | |
| P1282 | 5′-GCCTTATGCTTTTTCACAGCATCCATCTGTCCGCCCAGACGCCC-3′ | |
| P1373 | 5′-GCGGTGGCCGGGATTGATATCGGGC-3′ | |
| P1374 | 5′-CATCTGTCCGCCCAGACGCCCGATAT-3′ | |
| P1392 | 5′-GGCGGGGCAGCTGCCAATGGGCGTCTGGGCGGACAGATG-3′ | |
| P1393 | 5′-CATCTGTCCGCCCAGACGCCCATTGGCAGCTGCCCCGCC-3′ | |
| P1509 | 5′-GGTCCGCAGTGATGCCGGTGGGCGCGCCGACC-3′ | |
| P1510 | 5′-GGTCGGCGCGCCCACCGGCATCACTGCGGACC-3′ | |
| P1511 | 5′-GCCGATAACCAGAAAGGGTGGGCGCGCCGACC-3′ | |
| P1512 | 5′-GGTCGGCGCGCCCACCCTTTCTGGTTATCGGC-3′ | |
| P1513 | 5′-GCGAAGCCTCAGCCGGGGTGGGCGCGCCGACC-3′ | |
| P1514 | 5′-GGTCGGCGCGCCCACCCCGGCTGAGGCTTCGC-3′ |
a
Restriction enzyme sites are in boldface type.