FIG. 3.

Results of EIA using His-tagged full-length LcrE and His-tagged LcrE minus the C-terminal 50 amino acids (truncated LcrE). Both His-tagged proteins were used to coat a microtiter plate to which GST-LcrH-2 and GST alone were added, incubated, washed, and reacted with anti-GST MAb. Shown are the averages (bars represent ± standard deviations) of five reactions each between GST-LcrH-2 and His-LcrE and the truncated His-LcrE. The background binding for both reactions using His-LcrE (0.3 ± 0.01) and truncated His-LcrE (0.64 ± 0.12) was subtracted by using the average of five determinations using GST alone. Differences between the two reactions were statistically different (P < 0.001).