TABLE 1.

Plasmids and primers

Plasmid		Purposea	Forward primerb 5′-3′	Reverse primerb 5′-3′
Vector	Insert
pBT	Cpn0325	Bait, sycE	AAAGCGGATCCATGCAAAACCAATACGAGC	GTATTCCGCTCGAGTTACGCGACGTAGTAGATTCC
pBT	Cpn1021	Bait, lcrH-2	CTACGAATTCAATGTCACATTTAAATTATT	GCGGATCCTTATTTATGTTTTCGAATATC
pBT	Cps0740	Bait, lcrH-2	AACGCGGATCCATGTCATACTTAGCTTATTTAT	AAAGTTCTCGAGTTATGCGTGGTTTTTTATATC
pBT	CT862	Bait, lcrH-2	AGAATTCAATGCCACCAAGCAAGATCCAATGT	ATAGGATCCTTAGGCGTACGATTTGATCGC
pTRG	Cpn0324	Target, lcrE	ATAGGGGATCCATGGCAGCATCAGGAGGC	TCGGCCTCGAGTTATGACCAAGGATAGGGT
pTRG	Cps0458	Target, lcrE	AGGATCCATGGCTGCATCTGGAGGAGCT	ATAGAATTCTTAAGACCAGGGATAAGGTTT
pTRG	CT089	Target, lcrE	AGGATCCATGACTGCATCAGGAGGAGCTGGAG	ATAGAATTCTTAGGGTGATGGAGGCTGTGT
pTRG	Cpn0705	Target, HP	ATCAGGGCGAGCGGCCGCAATGGAATTAAAGAAAA	CGGCTCGAGTTATAAACGTGCTTCGAT
pTRG	Cpn0708	Target, HP	TTTGGATCCATGATAGACCCTGTAGAATGT	GCCCTCGAGTTAACCTAATTGCGTTGTCAT
pTRG	Cpn0808	Target, HP	AGGAGAATTCAGATGCCGCAATCAGCGGAG	GCCCTCGAGTTAATTAAAGATAGCAGATGT
pTRG	Cpn0809	Target, HP	GATGGATCCATGTCTATTTCATCTTCTT	GCGAATTCTTAAAATTATTGGTTTTATGTGC
pTRG	Cpn0810	Target, HP	GGAGGATCCATGAATAAAAAGCCCAAGA	GCGAATTCTTACTCAGCGCCTTTAACCAT
pTRG	Cpn1016	Target, HP	GAGAGGGATCCATGAAAAAAGGGAAATTAGCA	GCGAATTCTTACGAAGCAGAAGTCGTTGT
pTRG	Cpn1017	Target, HP	CCGTAGGATCCATGAGAAAACTTATTTTAT	ATGCCTCGAGTTAAGAACAACGGAGTTCT
pTRG	Cpn1018	Target, HP	TGCTGGGATCCATGTGGTATTTCAATTACC	GCCCTCGAGTTAATTAAAGATAGCAGATGT
pTRG	Cpn1019	Target, HP	CTTCTGGATCCATGACTGTATCTTACCAAT	GCCTCGAGTTACTGATACAGACTGCGAGC
pTRG	Cpn1020	Target, HP	CTTTGGGATCCATGTCCTCTTGCTTATCTC	GCGAATTCTTATCCTCTTAAGGAGTGGAG
pTRG	Cpn1022	Target, HP	TTATAGGATCCATGAATATGCCTGTTCCTTC	GCGAATTCTTAGGGGCGTAGGTTGTAAAT
pTRG	Cpn0324A	Target, −50N lcrE	AGGATCCCTGACAAATCCCGCAGCAGCAA	TCGGCCTCGAGTTATGACCAAGGATAGGGT
pTRG	Cpn0324B	Target, −100N lcrE	AGGATCCGCTTCTGGGAATTCTGAAATCT	TCGGCCTCGAGTTATGACCAAGGATAGGGT
pTRG	Cpn0324C	Target, −150N lcrE	AGGATCCCAAACGACTCCACCCTCCCAAG	TCGGCCTCGAGTTATGACCAAGGATAGGGT
pTRG	Cpn0324Z	Target, −50C lcrE	ATAGGGGATCCATGGCAGCATCAGGAGGC	ACTCGAGTTAAGAAAAGAATAAGTTCAAGACA
pTRG	Cpn0324Y	Target, −100C lcrE	ATAGGGGATCCATGGCAGCATCAGGAGGC	ACTCGAGTTAAGGAGTTTGGATTCCCTCAGCT
pTRG	Cpn0324X	Target, −150C lcrE	ATAGGGGATCCATGGCAGCATCAGGAGGC	ACTCGAGTTATTTTAATTCTGTTGCCATTCCT
pET-45b(+)	Cpn0324	His6-rLcrE	GCGGATCCGGCAGCATCAGGAGGCACA	TCGGCCTCGAGTTATGACCAAGGATAGGGT
pET-45b(+)	Cpn0324T	His6-rLcrE, −50C	TTGGATCCGGCAGCATCAGGAGGCACA	ACCCTCGAGTTAAGAAAAGAATAAGTTCAAGAC
pGEX-4T-1	Cpn1021	GST-rLcrH-2	GCGGATCCATGTCACATTTAAATTAT	AGAATTCTCATTTATGTTTTCGAATAT
pBT		Empty bait	TCCGTTGTGGGGAAAGTTATC	GGGTAGCCAGCAGCATCC
pTRG		Empty target	CAGCCTGAAGTGAAAGAA	ATTCGTCGCCCGCCATAA
pGEX-4T-1		GST vector	GGGCTGGCAAGCCACGTTTGGTG	CCGGGAGCTGCATGTGTCAGAGG
pET-45b(+)		His6 vector	TAATACGACTCACTATAGGG	GCAGCAGCCTAGGTTAATT

^aHP, hypothetical protein. −50N and −50C (and analogous designations), 50 aa deleted from the N and C termini, respectively, of the protein. r, recombinant.

^bBases underlined are the restriction endonuclease site used for cloning into the multiple cloning site of the plasmid vector.