TABLE 2.
β-Galactosidase activity of the C. pneumoniae lcrE deletion mutants in the two-hybrid bacterial system, using LcrH-2 as a bait protein
| LcrE> | β-Galactosidase activityb |
|---|---|
| Complete protein | 141 (4.3) |
| −50 aa, N terminus | 133 (1.2) |
| −100 aa, N terminus | 230 (7.8) |
| −150 aa, N terminus | 195 (4.8) |
| −50 aa, C terminus | 33 (0.4) |
| −100 aa, C terminus | 22 (0.3) |
| −150 aa, C terminus | 15 (0.9) |
| No proteina | 18 (3.3) |
a
lcrE was omitted from the target plasmid; therefore, cotransformants contained the bait plasmid with the lcrH-2 insert and the target plasmid minus lcrE.
b
β-Galactosidase activity is given in Miller units, with the standard deviation shown in parentheses.