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(A) In vitro transcription assay with the promoter region of lmo0596. RNAP of L. monocytogenes was loaded with different amounts of purified SigB protein, as indicated. (B) The corresponding in vitro transcripts were taken for primer extension to determine the transcriptional start sites. The putative −35 and −10 boxes of the SigB-recognized promoters are indicated. (C) The transcription start site is shown in bold letters.
