TABLE 1.
PCR primers useda
| Sequence (5′→3′) | Application |
|---|---|
| GAGGAATACAGGGTACCATGAGTGATAAAACAAAAAACACAAAAC; GGTTTGTCC TCCTGCAGGGTTGTGCATTATTCCAGGAAG | Construct for His6-tagged SigA |
| AAAAGCAGGTGGGGTACCATGCCAAAAGTATCTCAACC; TTTGATTCAACTGCAGT GTTCATTTACTCC | Construct for His6-tagged SigB |
| AAGTGGGGAATTCTGTATTTATTATGTC; TTTGGCGCTGCAGAAGCATCGATCTC | pUC18*PrsbV, template for in vitro transcription |
| GTATTGTGGATCCAACTAAAGTAACACG; CCATCACTGCAGCAATACCAATAAGTG | pUC18*Pbsh, template for in vitro transcription |
| AAGGATTGGTACCTTTTCCGACTTATC; ATAATCACTGCAGTCCCCGCTAATAA | pUC18*Plmo0596, template for in vitro transcription |
| AATCCGTTTCTAGATATGTATGC; ATACAATAAGCTTGTGGATCCCA | pUC18*PprfA, template for in vitro transcription |
| CAACAAATATGTCTATGTGGTC | Primer extension of PrsbV |
| CGGCGTAACAACCACAACTTC | Primer extension of Pbsh |
| CCGCTAATAAAACAAGAATTCGTG | Primer extension of Plmo0596 |
| GGTTTTATCCCGTTAG | Primer extension of PprfA |
a
Primers were used for His6-tagged SigA and SigB constructs and cloning of in vitro transcription templates. Oligonucleotides were used for primer extension. Restriction sites that were used are underlined.