Fig. 4.

Proliferative capacity of IL-6R^hiTIGIT⁻ mTregs is dependent on exogenous IL-2. Proliferative capacity of sorted (i) IL-6R^hiTIGIT⁺ (depicted in green), (ii) IL-6R^hiTIGIT⁻ (depicted in red), (iii) IL-6R^loTIGIT⁺ (depicted in blue) mTregs, and (iv) CD127⁺CD25⁻CD45RA⁻ Teff cells (depicted in black) was assessed in response to in vitro stimulation with anti-CD3/CD28 beads, in the absence of exogenous IL-2. Data (mean +/− SEM) were obtained from cells sorted from three independent donors. Proliferation and suppressive capacity were calculated using the Division Index in FlowJo, setting 0% suppression as the condition with the respective Teffs cultured in the absence of Tregs.