Fig. 7.

ex vivo isolated IL-6R^hiTIGIT⁺ mTregs show an activated Treg transcriptional profile. Volcano plot depicts the differential expression of 579 immune genes in IL-6R^hiTIGIT⁺ and IL-6R^hiTIGIT⁻ mTregs sorted ex vivo from nine independent healthy donors using NanoString. Plots depicting the normalized read counts from four differentially expressed activated Treg signature genes (marked in green), HELIOS, HLA-DRA, DUSP4 and FOXP3 are also shown. The flow sorting marker TIGIT, used for the isolation of the assessed Treg subsets, is marked in black. P values were calculated using two-tailed paired non-parametric Wilcoxon signed rank tests, comparing the normalized NanoString read counts between IL-6R^hiTIGIT⁺ and IL-6R^hiTIGIT⁻ mTregs.