Fig. 9.
IL-6RhiTIGIT+ mTregs chemokine receptor profile is consistent with a tissue-homing Treg subset. (A) Gating strategy for the delineation of the Th1, Th2, Th17 and Th22 Treg subsets. The delineation of the Th Treg subsets was based on the definition previously described by Duhen et al. [5]. (B) Data shown depicts the frequencies (GeoMean +/− 95% CI) of the Th subsets in CD45RA− IL-6R−/intTIGIT−; IL-6RhiTIGIT− and TIGIT+ CD127lowCD25+ T cells; and in memory T effector cells. (C) Data shown depicts the frequency of the individual chemokine receptors and cell adhesion markers in the same T cell subsets. P values were calculated using a two-tailed paired non-parametric Wilcoxon signed rank test, comparing the frequency of the assessed phenotypes between IL-6RhiTIGIT− mTregs and the other T cells subsets. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 10−4.