The groEL100 and ΔdinB::Zeo mutant alleles are epistatic in recG+ but not in recG mutant strains. Shown is the accumulation of Lac+ revertants of groE+, ΔdinB::Zeo, groEL100, and groEL100 ΔdinB::Zeo strains during incubation on lactose-minimal medium. Data are the cumulative number of Lac+ colonies divided by the number of viable Lac− cells on the plate 2 days earlier. Because it takes 2 days for a Lac+ revertant to make a visible colony, the values shown are displaced 2 days earlier. Each point is the mean ± 95% confidence limits of results for six cultures (some error bars are smaller than the symbols). (A) Closed circles, PFG60 (groE+); open circles, PFG250 (ΔdinB::Zeo); closed triangles, PFG61 (groEL100); open triangles, PFG251 (ΔdinB::Zeo groEL100). (B) Closed circles, PFG62 (groE+); open circles, PFG305 (ΔdinB::Zeo); closed triangles, PFG63 (groEL100); open triangles, PFG307 (ΔdinB::Zeo groEL100). For clarity, the results for PF62 after day 1 are not shown; see Fig. 2 for comparable data for this strain. All strains are also purA::Tn10.