TABLE 1.
E. coli strains and plasmids used in this study
| Strain(s) | Relevant phenotype or genotype | No. of copies of dinB+ | Reference |
|---|---|---|---|
| FC29 | ara Δ(gpt-lac)5 thi/F′ Δ(lacIZ) Pro+ | 2 | 6 |
| FC36 | ara Δ(gpt-lac)5 thi Rifr | 1 | 6 |
| FC40 | FC36/F′ Φ(lacI33-lacZ) Pro+ | 2 | 6 |
| FC526 | FC40 ΔrecG263::Kn | 2 | 20 |
| FC1230 | FC40/pYG768 | Many | This study and reference 29 |
| FC1240 | FC722 ΔdinB::Kn on chromosome and episome | None | 34 |
| PF2244 | TE8197a Arg+ Tets | 1 | This study and reference 26 |
| PF2245 | TE8222b Arg+ Tets | 1 | This study and reference 26 |
| PF2252 | WAM106c PL-lacZ | 1 | 61 |
| PF2253 | WAM106c PgroE-lacZ | 1 | 61 |
| PF2258 | PF2252 rpoS::Cm | 1 | This study and reference 34 |
| PF2259 | PF2253 rpoS::Cm | 1 | This study and reference 34 |
| PFG60, PFG64d | FC40 groEL+purA::Tn10 | 2 | This study |
| PFG61 | FC40 groEL100 purA::Tn10 | 2 | This study and reference 66 |
| PFG62, PFG66d | FC526 groEL+purA::Tn10 | 2 | This study |
| PFG63 | FC526 groEL100 purA::Tn10 | 2 | This study |
| PFG65 | FC40 groES30 purA::Tn10 | 2 | This study and reference 33 |
| PFG67 | FC526 groES30 purA::Tn10 | 2 | This study |
| PFG250 | FC40 groE+purA::Tn10 ΔdinB::Zeo on chromosome and episome | None | This study and reference 5 |
| PFG251 | FC40 groEL100 purA::Tn10 ΔdinB::Zeo on chromosome and episome | None | This study |
| PFG305 | FC526 groE+purA::Tn10 ΔdinB::Zeo on chromosome and episome | None | This study |
| PFG307 | FC526 groEL100 purA::Tn10 ΔdinB::Zeo on chromosome and episome | None | This study |
| PFG308 | FC36 sulA-lacZ groEL+purA::Tn10 | 1 | This study |
| PFG309 | FC36 sulA-lacZ groEL100 purA::Tn10 | 1 | This study |
| PFG332 | PFG308 recG263::Kn | 1 | This study |
| PFG333 | PFG309 recG263::Kn | 1 | This study |
| PFG324 | FC36 sulA11 groEL+purA::Tn10 lexA71::Kn | 1 | This study and reference 34 |
| PFG325 | FC36 sulA11 groEL100 purA::Tn10 lexA71::Kn | 1 | This study and reference 34 |
| PFG326 | FC36 sulA11 groEL+purA::Tn10 | 1 | This study and reference 34 |
| PFG327 | FC36 sulA11 groEL100 purA::Tn10 | 1 | This study and reference 34 |
| PFG328 | PFG324/F′ Φ(lacI33-lacZ) Pro+ | 2 | This study |
| PFG329 | PFG325/F′ Φ(lac/33-lacZ) Pro+ | 2 | This study |
| PFG330 | PFG326/F′ Φ(lacI33-lacZ) Pro+ | 2 | This study |
| PFG331 | PFG327/F′ Φ(lacI33-lacZ) Pro+ | 2 | This study |
| PFG334 | GJ2770egroEL+purA::Tn10 | 1 | This study |
| PFG335 | GJ2770egroEL100 purA::Tn10 | 1 | This study |
| PFG336 | PFG334 rpoS::Cm | 1 | This study |
| PFG340 | PF2244 groEL+purA::Tn10 | 1 | This study |
| PFG341 | PF2244 groEL100 purA::Tn10 | 1 | This study |
| PFG342 | PF2245 groEL+purA::Tn10 | 1 | This study |
| PFG343 | PF2245 groEL100 purA::Tn10 | 1 | This study |
| PFG321 | FC40 groES::Cm | 2 | This study |
| PFG351 | FC526 groES::Cm | 2 | This study |
a
TE8197 is MG1655 Δ(lacIZ. argA::Tn10 trpDC700::putPA1303::Knr-rpoS-lac (protein fusion) (26). MG1655 is considered to be a wild-type strain (4).
b
TE8222 is MG1655 Δ(lacIZ. argA::Tn10 trpDC700::putPA1303::Knr-rpoS-lac (operon fusion) (26).
c
WAM106 is F− araD139 Δ(argF-lac)U169 Δ(his-gnd. thi rpsL150 flbB5301 relA1 deoC1 rbsR (57).
d
Both strains were constructed by transduction; the donor for the first was GW7503 (=AB1157 groEL100 purA::Tn10), and the donor for the second was GW7504 (=AB1157 groES30 purA::Tn10) (9). Tetr Ade− λvir-sensitive transductants were isolated.
e
GJ2770 is MC4100 λ [katE::lac(Kan)] (47). MC4100 is Δ(argF-lac)U169 rpsL150 relA1 araD139 flbB5301 deoC1 ptsF25.