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Western immunoblot analysis of wild-type (WT), mutant (III-4), revertant (III-4-R1), and transformant M. pneumoniae lysates. Two independent transformants are shown for each transposon-background combination. Forty micrograms of protein was used per lane. Anti-P90 (upper) and anti-P40 (lower) sera were used at a dilution of 1:1,000.
