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S1 nuclease protection analysis of actII-ORF4, redD, redZ, and hrdB (positive control) transcription during development of S. coelicolor M145 and J2168 (M145ΔbldC) on R2YE solid medium. The time points in hours at which mycelium was harvested for RNA isolation and the presence of vegetative mycelium (V), aerial mycelium (A), and spores (S) are shown, as judged by microscopic examination.
