TABLE 1.

Strains and oligonucleotides used in this study

Strain or primer	Genotype or sequence	Reference or source
Strains
S. coelicolor
M600	SCP1− SCP2−	28
M145	SCP1− SCP2−	28
J660	bldC18 mthB2 cysD18 agaA7 SCP1NF SCP2*	34
J2161	ΔbldC::apr derivative of M600	This work
J2162	ΔbldC::apr derivative of J660	This work
J2166	ΔbldC derivative of M600	This work
J2167	ΔbldC::apr derivative of M145	This work
J2168	ΔbldC derivative of M145	This work
J2169	bldC+ derivative of J660	This work
J3210	bldC18 derivative of M600	This work
J3211	bldC18 derivative of M145	This work
J1700	bldA39 hisA1 uraA1 strA1 SCP1− SCP2−	30
J669	bldB43 mthB2 cysD18 agaA7 SCP1NF SCP2*	34
J774	bldD53 cysA15 pheA1 mthB2 strA1 SCP1NF SCP2*	34
166	bldF hisD3 pheA1 strA1 SCP1+ SCP2+	43
WC103	bldG103 hisA1 uraA1 strA1 Pgl−SCP1− SCP2−	7
WC109	bldH109 hisA1 uraA1 strA1 Pgl−SCP1− SCP2−	7
HU261	bldJ261 hisA1 uraA1 strA1 Pgl− SCP1NF SCP2*	50
NS17	bldK::aadA derivative of M145	39
J2151	ΔglkA119 bldM::hyg SCP1− SCP2−	35
J2177	ΔglkA119 bldN::hyg SCP1− SCP2−	2
Primers
Cloning and mutagenesis primers
BLDC1	CGTCGCTTGGAGCTGTGGTTCC
BLDC2	TGCCGACAAGGGACGTAGGTCC
BLDC5	CCGCATATGACCGCTCGTACCCCGGACGCTGAGCCGCTGCTGACC
BLDC6	CCCGGTTATTCAGTTGTTCAGG
Y49F FOR	TTCCGCGAAGCCGAGGTCCG
Y49F REV	GCGGCGATGCCCGCCGAGCG
Gene disruption primers
BLDC K-OUT FOR	ATGAACCGAGAAGGTTCGGTTCTCCCGAGGAGGCCGCTCATTCCGGGGATCGTCGACC
BLDC-K-OUT REV	GGTTGGGGGACCCGCCGTTCTGCCCGGTTATTCAGTTGTTGTAGGCTGGAGCTGCTTC
S1 nuclease mapping and runoff primers
BLDC2	See cloning and mutagenesis primers
BLDC3a	GAACATGGTGGCGACCTCAGCC
BLDC4	TATATACTGTGACTGATCGTCACAGAGG
BLDC17	GAGCGGCCTCCTCGGGAG
BLDC25a	GCACCTTGACCCGCGTTGCC
ACTIIORF4.1a	GTACGTCTGCAGCGTCGTCATGGCGCT
ACTIIORF4.2	ATCGGAGATCGCTTGTGACGGCA
HRDB FOR	CGCAAGGTACGAGTTGATGACC
HRDB REVa	CCATGACAGAGACGGACTCGG
REDD.1a	GATCGATACGGGTCCCAATA
REDD.JW2	CATGGATCCTGCTTCGTTTGCGTCGTTCAGTTC
REDZ.1a	CTCCAGCAATGCGCGAAT
REDZ.2	CGTGCACGTGTCCTTCTG

^aLabeled primers.