Figure 8.
Trafficking of the amino-terminal fragment of apoE4 to the nucleus confirmed by nuclear fractionation. Separation of nuclear, cytoplasmic and mitochondrial proteins by differential centrifugation was undertaken following treatment of BV2 cells with apoE1-151 (50 µg/ml) for 24 hours. The upper panel in each section shows immunoblotting results following Western blot analysis utilizing an anti-His antibody showing the presence of the apoE41-151 faintly in the cytoplasm with much stronger labeling found in the nuclear and mitochondrial fractions (lanes marked ‘2’). MEK1/2 was employed as a cytoplasmic marker, Histone H3 as a nuclear marker, Rab5 as an endosomal marker, and AIF as a mitochondrial marker. Results are representative of two independent experiments.