Figure 3.

AOAA treatment prevented LPS-induced increases in the protein levels of COX-2 of the BV2 microglia. A. Representative Western blots showing the effect of AOAA on the COX-2 level of the BV2 cells. B. Quantifications of the Western blots indicated that 0.5 mM AOAA treatment prevented 1 μg/mL LPS-induced increase in the COX-2 level of the cells. The BV2 microglia were pre-treat with 0.5 mM AOAA for 0.5 h, followed by co-treatment with 1 μg/mL LPS for 23.5 h. The protein level of COX-2 of the cells was determined by Western blots. Data were collected from three independent experiments. N = 6. ***P < 0.001.