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. 2017 May 17;7:2002. doi: 10.1038/s41598-017-02016-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of shRNA library screening using etoposide. (A) HeLa S3 cells were infected with Module 1 and split into two subpopulations, one of which was treated with 0.2 μM etoposide for 6 days. Relative read counts of each barcode were compared between treated and untreated cells. shRNAs against topo IIα are highlighted in red. (B) HeLa S3 cells were transfected with siRNA pools against 6 candidate genes, and then cultured for 2 days in the presence of the indicated concentrations of etoposide. Cell viability was measured by WST-8 assay. Data represent means ± SD from three independent experiments.