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. 2017 May 17;7:2002. doi: 10.1038/s41598-017-02016-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combined treatment of aurilide B with ouabain. (A) Cells were treated for 24 h with the indicated concentrations of aurilide B or ouabain. OPA1 was analyzed by Western blotting. α-tubulin served as a loading control. (B,C) Cells were treated for 4 h (B) or 24 h (C) with the indicated concentrations of aurilide B or ouabain. Cellular ATP content was measured by ATPlite. For the 24-h samples, data were normalized against protein content. Data represent means ± SD from three independent experiments. (D) Cells were treated for 24 h with the indicated concentrations of aurilide B or ouabain. Cleaved PARP was analyzed by Western blotting. Uncropped blots are found in Supplementary Fig. S4.