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. 2017 May 17;7:2017. doi: 10.1038/s41598-017-01666-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of corticosterone exposure (Cort-black bars) on (A) maternal blood glucose concentrations, (B) maternal food consumption, (C) hepatic mRNA expression of solute carrier family 2, facilitated glucose transporter member 1 (Slc2a1 or Glut1), (D) Glutamine-fructose-6-phosphate-transaminase 1 (Gfpt1) and (E) O-linked β-N-Acetylglucosamine (OGT) compared to untreated (Untr-white bars) controls. Protein expression of hepatic OGT was also investigated in Untr or Cort exposed dams (F). Data represented as mean + SEM. Maternal blood glucose concentrations and hepatic gene expression results were analysed by two-tailed t-tests (n = 6–7). Maternal food consumption was measured daily from E11.5 until E15.5 and data analysed using a one way ANOVA. Protein expression was examined by western blot with β-Actin (ACTB) as a loading control, normalised to untreated control and analysed using a two-tailed t-test.