Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 17;7:2017. doi: 10.1038/s41598-017-01666-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The effects of corticosterone exposure (black bars) on (A) serine 473 phospho-AKT1, (B) serine 474 phospho-AKT2 along with (C) total AKT1 and (D) total AKT2 protein in whole placentae of male and female C57/BL6 mouse fetuses at E14.5 compared with untreated controls (white bars). (E) Phosphorylation of AKT1 on threonine 308 relative to total AKT1 was also examined. Pooled male and female E14.5 placental tissue (n = 6) was further immunoprecipitated with RL2 antiserum to isolate O-GlcNAcylated proteins and immunoprobed with pan Akt antiserum following SDS-PAGE (F). Data represents mean + SEM, **P < 0.01. Protein expression examined by western blot with β-Actin (ACTB) as loading control, normalised to untreated control of the same sex (Top band analysed for tAKT and both bands analysed for O-GlcNAcylated AKT). Analysed using a two-tailed t-test with untreated controls of the same sex (n = 6).