Table 1.
Antibodies used for immunoblotting and precipitation.
| Full Name | Abbreviation | Raised in | Company | Product No. | Dilution |
|---|---|---|---|---|---|
| Glutamine fructose-6-phosphate transaminase 1 | GFPT1 | rabbit | Abcam | Ab125069 | 1:1000 |
| O-linked N-acetylglucosamine (GlcNAc) transferase | OGT | rabbit | Cell Signalling Technology (CST) | 5368S | 1:1000 WB |
| 1:25 IP | |||||
| O-linked N-acetylglucosamine | O-GlcNAc (CTD110.6) | mouse | CST | 9875S | 1:1000 |
| O-linked N-acetylglucosamine | O-GlcNAc (RL2) | mouse | Abcam | ab2739 | 1:000 WB |
| 1:20 IP | |||||
| Heat Shock Protein 90 | HSP90 | rabbit | CST | 4877S | 1:1000 |
| Protein Kinase B 2 | AKT2 | rabbit | CST | 3063P | 1:800 |
| Serine474-phosphorylated Protein Kinase B2 | Phospho- AKT2(Ser474) | rabbit | CST | 8599S | 1:800 |
| Protein Kinase B 1 | AKT1 | mouse | CST | 2967 | 1:1000 |
| Serine473-phosphorylated Protein Kinase B1 | Phospho- AKT1(Ser473) | rabbit | CST | 4060 | 1:1000 |
| Threonine308-phosphorylated protein kinase B1 | Phospho-Akt (Thr308) | Rabbit | CST | 9275 | 1:1000 |
| Glucocorticoid receptor (D8H2) | GCR | Rabbit (mAb) | CST | 3660P | 1:000 |