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. 2017 May 17;7:2018. doi: 10.1038/s41598-017-02291-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Taq polymerase stop assay of lentiviral G4 sequences. Oligonucleotides bearing PQSs were folded in the absence (−) or presence (+) of KCl. KCl-treated samples were further incubated with the G4 ligand BRACO-19 (B). Oligonucleotides were used as templates in a Taq polymerase reaction at 47 °C or 37 °C as indicated. Symbols * indicate premature stop site at G bases in gel images (a) and in the corresponding G4 sequences (b). Symbols ^ indicate stop sites independent of G4 folding. G-bases are shown in bold.