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. 2017 May 17;7:2032. doi: 10.1038/s41598-017-02174-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology and proliferation rate detection of the parental tumour and the U-CH14 cell line. (a) Section of the parental tumour. The cells show the typical physaliphorous appearance. The tumour is classified as not otherwise specified (NOS) subtype. (b) Ki-67 staining of a parental tumour section. The proliferation index is below 5%. (c) Phase contrast image of the U-CH14 cell line. The typical morphology is conserved in vitro. (d) Proliferation index of U-CH14 (<5%; Ki-67 staining). Lines indicate 200 µm (a+b) or 100 µm (c+d).