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. 2017 May 17;7:2032. doi: 10.1038/s41598-017-02174-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of several HOX gene family members. (a) Box plot illustration of the differences in the expression of HOXA2, -A4, -A5, -A7, -A9, -A10, -B3, -B7, and -C9 between clivus (c) and sacrum (S) chordoma cell lines. Measurements represent the results of microarray expression profiling of three clivus and nine sacrum chordoma cell lines (each cell line was tested at least in duplicates). (b) Validation of the expression of HOXA7, -A9 and -A10 in the chordoma cell lines via qPCR shown as measured ΔCT values. High ΔCT values represent lower expression of the corresponding gene. Measurements were performed in triplicates. Differences in expression were tested by t-tests. p < 0.05 was regarded as significant difference (*). **p < 0.01.