Table 2.
Stability values and ranking order of seven candidate reference genes obtained of all analysed samples from Idared and Free Redstar based on results from geNorm, NormFinder, BestKeeper, Delta Ct and Comprehensive ranking.
| Ranking | Genorm | NormFinder | BestKeeper | Delta Ct | Comprehensive ranking | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| gene | M-value* | gene | Stability value* | gene | Std dev [ ± CP]* | gene | Average of st dev* | Gene | Geomean of ranking values (GM)* | |
| 1 | proC | 0.550 | proC | 0.077 | ffh | 0.46 | proC | 0.55 | proC | 1.41 |
| 2 | recA | 0.558 | pykA | 0.091 | pykA | 0.48 | recA | 0.56 | recA | 2.06 |
| 3 | pykA | 0.561 | recA | 0.094 | recA | 0.49 | pykA | 0.57 | ffh | 2.63 |
| 4 | ffh | 0.603 | ffh | 0.104 | proC | 0.50 | ffh | 0.60 | pykA | 2.63 |
| 5 | gyrA | 0.631 | gyrA | 0.123 | gyrA | 0.57 | gyrA | 0.63 | gyrA | 5.23 |
| 6 | rpoB | 0.661 | rpoB | 0.135 | rpoB | 0.59 | rpoB | 0.66 | rpoB | 5.73 |
| 7 | glyA | 0.747 | glyA | 0.163 | glyA | 0.67 | glyA | 0.75 | glyA | 7.00 |
*As lower value as more stable gene.