Table 2.

Studies utilizing cDNA microarray technology to study asthma

Investigator	Microarray type	Species/Sample size	Summary/Key findings	Normalization procedure
	Number of genes	Type of tissue		Replications per data point
Zou et al.32	cDNA 40.000 elements	10 monkeys Lung tissue samples	Microarray profile of differentially expressed genes in a monkey model of allergic asthma.	Ratios of Cy5/Cy3 multiplied to the balance coefficient of the microarray / 3 replicates
Brutsche et al.33	cDNA 600 genes	40 subjects Mononuclear cells	CAGE score for atopy and asthma.	Absolute difference of the expression of CAGE scored genes 1 replicate
Sayama et al.38	cDNA 14.000 genes	human umbilical cord mast cells	Transcriptional response of human mast cells stimulated via the Fc (epsilon) RI and identification of mast cells as a source of IL-11.	Array-specific normalization coefficient was calculated by centering in log base 2 space a dataset consisting of all elements with an I/D> 3-fold / 2 replicates
Brutsche et al.41	cDNA 600 genes	40 subjects Mononuclear cells	Apoptosis signals in atopy and asthma measured with cDNA arrays	G.I was normalized to the housekeeping G.I / 1 replicate
Syed et al. 42	cDNA 12.228 genes	Human CD4+ T cells	CCR7 down-regulation in asthma	Median G.I of each filter normalized any differences in cDNA probe activity between filters/ 1 replicate
Banerjee et al.43	cDNA 1.176 genes	18 mice Lung tissue samples	Gene expression profiling in inflammatory airway disease associated with elevated adenosine	G.I was normalized to the housekeeping G.I / 2 replicates

Abbreviations: CAGE: Composite atopy gene expression, CCR7: Chemokine receptor 7, G.I: Gene Intensity, I/D: Intensity/Background ratio, Th: T helper, RI: Immunoglobulin receptor