Table 5.
Studies utilizing microarray technology to study ALI/PE
| Investigator | Microarray type | Species/Sample size | Summary/Key findings | Normalization procedure |
| Number of genes | Type of tissue | Replications per data point | ||
| McDowell et al.56 | cDNA 8.374 genes | 6 mice Lung tissue samples | Differential gene expression in the initiation and progression of nickel-induced ALI. | Ratios of Cy5/Cy3 multiplied to the balance coefficient of each microarray / 5 replicates |
| Olman et al.58 | cDNA 588 genes | 36 patients Pulmonary edema Lung fibroblasts | Microarray analysis indicates that pulmonary edema fluid from patients with ALI mediates inflammation, mitogen gene expression, and fibroblast proliferation through bioactive IL-1. | G.I normalized to the housekeeping G.I 2 replicates |
| Kupfner et al.61 | Oligonucleotide | Mice Lung neutrophils | Role of NF-κB in endotoxemia-induced alterations of lung neutrophil apoptosis. | Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 3 replicates |
| Cher et al.62 | Oligonucleotide 8.800 genes | 21 rats Whole lungs | Pulmonary inflammation and edema induced by Phospholipase A2. | Each gene was divided by the median of its values in all samples / 3 replicates |
| Sabbadini et al.63 | Oligonucleotide 12.600 genes | 14 rabbits Lung tissue samples | Gene expression analysis in interstitial lung edema induced by saline infusion. | Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 2 replicates |
| Perkowski et al.64 | cDNA 8.374 genes | 20 mice Lung tissue samples | Gene expression profiling of the early pulmonary response to hyperoxia in mice. | Difference between observed log-ratio and corresponding fitted ratio/ 5 replicates |
| Ward et al.65 | cDNA 7.398 genes | 6 rats Lung and other organ samples | Molecular signatures of sepsis: multiorgan gene expression profiles of systemic inflammation. | Gene expression levels normalized by a scaling factor multiplied to the average of differences of probe pairs / 4 replicates |
Abbreviations: ALI: Acute Lung Injury, G.I: Gene Intensity, PE: Pulmonary Edema, NF-κB: Nucleus Factor-κB