Figure 6.
Cellular function of miR-520b occurs through modulation of CD44. Cell adhesion (A), invasion (B) and sphere formation (C) were examined in OECM1 ad SAS cells as described in the Methods section. (D) EMT and cancer stemness associated protein expressions were determined by western blot analysis. Actin protein levels were used as an internal control. The relative density of each sample was determined after normalization to the actin level. All the experiments were performed three times independently and that typical results were shown. The error bars shown in the relevant figures indicated the standard deviation of the quantification results in all experiments. (*p < 0.05, **p < 0.01, t-test).