Figure 6.

Increasing cytosolic Ca²⁺ is sufficient to attenuate thermogenic gene expression. (a) Oil red O staining of immortalized primary brown adipocytes stably transduced with ATP2A2 shRNA and immunoblot of ATP2A2, UCP1, PPARγ and GAPDH (loading control). (b) Measurement of cytosolic Ca²⁺ in immortalized brown adipocytes stably expressing shATP2A2. (c–f) mRNA levels of thermogenic genes (c), adipogenic/lipogenic genes (d), mitochondrial biogenesis genes (e) and mitochondrial-encoded ETC genes (f). Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired Student’s t-test (b–f).