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. 2017 May 17;7:1997. doi: 10.1038/s41598-017-01807-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ridge based microfluidic device for viable cell sorting. (a) Schematic representation of the device showing that cells will squeeze through ridges and flow towards different outlets based on their stiffness differences; (b) optical micrograph of the fabricated device showing the ridges and outlets; (c) representative micrograph of overlaid video of dead and live cells moving towards opposite directions in the device. The yellow arrow show the direction of fluid flow.