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. 2017 May 17;7:1997. doi: 10.1038/s41598-017-01807-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of the EthD-1 stained cells (a) cells in inlet; (b) cells collected from three different outlets of the device. The number of live and dead cells was calculated by comparing the results with control experiments; (c) flow cytometry data of control live and dead cells stained with Ethd-1 which is used to determine the threshold level to distinguish live and dead cells; (d) average Young’s modulus of sorted cells collected from three outlets (N = 20, *p-value < 0.01, and ***p-value < 0.001).