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. 2017 May 18;8:831. doi: 10.3389/fpls.2017.00831

FIGURE 2.

FIGURE 2

Confocal GFP fluorescence scanning of N-terminal tagged gene transgenic tobacco leaf cells. (A) GFP-CYP76AD1 and GFP-DODA1; (B) GFP-CYP76AD6 and GFP-cDOPA5GT. Every GFP construct and nucleolus-localized RFP marker or dual nucleus and cytoplasm RFP marker were co-transformed to tobacco (N. benthamiana) leaf epidermal cells by agro-infiltration, and 2 or 3 days post infiltration, the cells were assessed for fluorescence under Carl Zeiss 710 confocal laser scanning microscopy. For each enzyme, the upper panel shows red fluorescence from nucleolus marker RFP, the middle one, red fluorescence from chloroplast autofluorescence, and the lower one, dual nucleus and cytoplasm marker RFP. AD1, CYP76AD1; AD6, CYP76AD6; 5GT, cDOPA5GT; Nuc RFP, Nucleolus-localized RFP marker; Chl, Chloroplast autofluorescence; Nuc-cyt RFP, dual nucleus and cytoplasm RFP marker; GFP-Gene, GFP tagged at the N-terminus of the gene; Bar = 20 μm.