Table 1.

Preclinical studies with CAR NK-92 cells.

Target	Antibody	Hinge	TM	Signaling	Gene transfer	Cancer type	In vivo model	Treatment	Reference
ErbB2 (HER2)	FRP5	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	Breast ca. Ovarian ca. SCC	CD-1 nude	Local co-injection	Uherek et al. (23)
ErbB2 (HER2)	FRP5	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	Breast ca.	BALB/c nude	Systemic	Daldrup-Link et al. (63), Meier et al. (64)
ErbB2 (HER2)	FRP5	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	Brain metastasis	Athymic nude rats	Systemic (with FUS)	Alkins et al. (65), Alkins et al. (66)
ErbB2 (HER2)	4D5-8	hIgG2	hFcεRIγ	hFcεRIγ	Retrovirus	Breast ca.	NSG	Systemic	Clemenceau et al. (58)
ErbB2 (HER2)	FRP5	hCD8α	hCD3ζ hCD28 hCD137	hCD3ζ hCD28-CD3ζ hCD137-CD3ζ	Lentivirus	Breast ca. Ovarian ca. Melanoma RCC	NSG	Systemic	Schönfeld et al. (67)
ErbB2 (HER2)	n.s.	hCD8α	hCD28	hCD28-CD3ζ	Electroporation	Breast ca.	BALB/c nude	Systemic	Liu et al. (68)
ErbB2 (HER2)	FRP5	hCD8α	hCD28	hCD28-CD3ζ	Lentivirus	GBM	NSG C57BL/6	Local	Zhang et al. (69)
Epidermal growth factor receptor (EGFR)	528	n.s.	n.s.	hCD28-CD3ζ	Lentivirus	Brain metastasis	NSG	Local (combined with HSV-1)	Chen et al. (70)
EGFR	R-1	hCD8α	hCD28	hCD28-CD3ζ	Lentivirus	GBM	NSG	Local	Genßler et al. (71)
EGFR/EGFRvIII	528	n.s.	hCD28	hCD28-CD3ζ	Lentivirus	GBM	NSG	Local	Han et al. (72)
EGFR/EGFRvIII	225	hCD8α	hCD28	hCD28-CD3ζ	Lentivirus	GBM	NSG	Local	Genßler et al. (71)
EGFRvIII	MR1-1	hCD8α	hCD28	hCD28-CD3ζ	Lentivirus	GBM	NSG	Local	Genßler et al. (71)
GD2	ch14.18	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	NB Breast ca. Melanoma	NSG	Local	Esser et al. (56), Seidel et al. (59)
Epithelial cell adhesion molecule (EpCAM)	MOC31	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	Prostate ca.	Athymic nude rats	Systemic	Tavri et al. (55), Meier et al. (73)
EpCAM	MOC31	hCD8α	hCD28	hCD28-CD3ζ	Lentivirus	Breast ca.	–	–	Sahm et al. (28)
CD19	FMC63	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	B-ALL	–	–	Romanski et al. (48), Romanski et al. (53)
CD19	FMC63	mCD8α	mCD3ζ	mCD3ζ	mRNA transfection	B-ALL CLL	–	–	Boissel et al. (50)
CD19	FMC63	mCD8α	mCD3ζ	mCD3ζ	mRNA transfection Lentivirus	B-ALL	CLL	Burkitt’s lymphoma	–	–	Boissel et al. (51)
CD19	FMC63	mCD8α	mCD3ζ	mCD3ζ	Lentivirus	B-ALL CLL	NOD/SCID NSG	Local Systemic	Boissel et al. (52)
CD19	FMC63	hCD8α	hCD3ζ hCD28 hCD137	hCD3ζ hCD28-CD3ζ hCD137-CD3ζ	Lentivirus	B-ALL Burkitt’s lymphoma	NSG	Systemic	Oelsner et al. (74)
CD20	Leu-16	mCD8α	mCD3ζ	mCD3ζ	Retrovirus	B-ALL CLL Burkitt’s lymphoma	NSG	Local co-injection	Müller et al. (49)
CD20	Leu-16	mCD8α	mCD3ζ	mCD3ζ	mRNA transfection	B-ALL	CLL	Burkitt’s lymphoma	–	–	Boissel et al. (51)
Lentivirus
CD20	Leu-16	mCD8α	mCD3ζ	mCD3ζ	Lentivirus	B-ALL	NOD/SCID	Local	Boissel et al. (52)
CLL	NSG	Systemic
CD3	n.s.	hCD8α	hCD8α	hCD28-CD137-CD3ζ	Lentivirus	PTCL	NSG	Systemic	Chen et al. (75)
T-ALL
CD5	n.s.	hCD8α	hCD8α	hCD28-CD137-CD3ζ	Lentivirus	PTCL T-ALL CLL Burkitt’s lymphomaSézary syndrome	NSG	Systemic	Chen et al. (76)
CD138	4B3	hCD8α	hCD3ζ	hCD3ζ	Lentivirus	MM	NOD/SCID	Systemic	Jiang et al. (54)
CS1	Luc90	n.s.	n.s.	hCD28-CD3ζ	Lentivirus	MM	NSG	Systemic	Chu et al. (77)
EBNA3C peptide	EBNA Clone 315	hCD8α	hCD8α	hCD137-CD3ζ	Retrovirus	BLCL	–	–	Tassev et al. (78)
gp100209–217 peptide	GPA7	n.s.	HLA-A2	hCD3ζ	Electroporation	Melanoma	NOD/SCID	Systemic	Zhang et al. (57)
WT1126 peptide	Q2L	hCD8α	hCD8α	hCD137-CD3ζ	Retrovirus	B-ALL AMoL NB	–	–	Zhao et al. (79)

TM, transmembrane domain; n.s., not specified; m, murine; h, human; SCC, squamous cell carcinoma; RCC, renal cell carcinoma; GBM, glioblastoma; NB, neuroblastoma; B-ALL, B-cell acute lymphoblastic leukemia; CLL, chronic lymphocytic leukemia; PTCL, peripheral T-cell lymphoma; T-ALL, T-cell acute lymphoblastic leukemia; MM, multiple myeloma; BLCL, Epstein–Barr virus (EBV)-transformed lymphoblastoid B cell line; AMoL, acute monocytic leukemia; FUS, MRI-guided focused ultrasound.